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direct epifluorescent filter techniqu

The direct epifluorescent filter technique (DEFT

With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram-staining procedure using acridine orange as the counterstain. The method enumerated viable Gram-negative and all Gram-positive bacteria.

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The direct epifluorescent filter technique (DEFT

With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram‐staining procedure using acridine orange as the counterstain. The method enumerated viable Gram‐negative and all Gram‐positive bacteria.

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The direct epifluorescent filter technique (DEFT).

The direct epifluorescent filter technique (DEFT). Kroll R(1). Author information: (1)Torry Research Station, Aberdeen, UK. PMID: 7550701 [Indexed for MEDLINE] MeSH terms. Animals; Bacteriological Techniques* Colony Count, Microbial/methods; Filtration/instrumentation; Fluorescent Antibody Technique, Direct/methods* Food Microbiology; Milk

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The Direct Epifluorescent Filter Technique (DEFT

Pettipher, G. L. (1983) The Direct Epifluorescent Filter Technique for the Rapid Enumeration of Microorganisms. Research Studies Press, Letchworth, UK.

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The direct epifluorescent filter technique (DEFT

With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram‐staining procedure using acridine orange as the counterstain. The method enumerated viable Gram‐negative and all Gram‐positive bacteria.

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direct epifluorescent filter technique (DEFT) YouTube

Oct 10, 2019 food science and technology(CBE670) Detection method for food microbes credit to : https://youtube/watch?v=PCJ13LjncMc

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Review: The direct epifluorescent filter technique

Review: The direct epifluorescent filter technique Review: The direct epifluorescent filter technique PETTIPHER, G. L. 1986-04-01 00:00:00 The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the method

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Application of the direct epifluorescent filter technique

Jul 01, 1985 The direct epifluorescent filter technique (DEFT) and the standard plate count (SPC) were compared in the examination of raw and heat-treated meat products. The most promising results were obtained with raw ground beef, where good

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Collaborative trial of the direct epifluorescent filter

The direct epifluorescent filter technique (DEFT) is a new rapid method which uses membrane filtration and epifluorescent microscopy for counting in milk. A collaborative trial of the DEFT was conducted between six laboratories. Each laboratory obtained a highly significant relationship between the DEFT count and plate count with a correlation

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Application of the direct epifluorescent filter technique

Jul 01, 1985 International Journal of Food Microbiology, 2 (1985) 139-144 139 Elsevier JFM 00042 Application of the direct epifluorescent filter technique as a rapid method in microbiological quality assurance in the meat industry S. H. Qvist' and M. Jakobsen 2 Danish Meat Products Laboratory, Howistoej 13, 2000 Copenhagen, and = Alfred Jorgensen Laborato: y for Fermentation Ltd.,

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Collaborative trial of the direct epifluorescent filter

The direct epifluorescent filter technique (DEFT) is a new rapid method which uses membrane filtration and epifluorescent microscopy for counting in milk. A collaborative trial of the DEFT was conducted between six laboratories. Each laboratory obtained a highly significant relationship between the DEFT count and plate count with a correlation

More

Improved Direct Epifluorescent Filter Technique for Rapid

Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions. Jose Maria Esteban, Mariano Leiva Diaz, Fabian Soriano and Enrique Marin. PDA Journal of Pharmaceutical Science and Technology September 1992, 46 (5) 146-149; Article

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Review: The direct epifluorescent filter technique

The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the method are discussed.

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A modified direct epifluorescent filter technique for the

A modified direct epifluorescent filter technique (DEFT) for the detection and enumeration of visible yeast in fruit‐flavoured yoghurt is described. The method involves an initial enrichment in oxytetracycline glucose yeast peptone broth (OGYP, 30d̀C/24, 48 h), prefiltration prior to DEFT and use of the vital stain Viablue 1. Additional yoghurt samples were subjected to prolonged incubation

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Comparison of antibody-direct epifluorescent filter

The antibody-direct epifluorescent filter (Ab-DEFT) technique was evaluated as a rapid alternative to the most probable number (MPN) method for enumeration of artificially inoculated Listeria monocytogenes in ready-to-eat packaged salads and other fresh vegetables.

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Use of the Direct Epifluorescent Filter Technique for the

Use of the Direct Epifluorescent Filter Technique for the Enumeration of Viable and Total Acetic Acid Bacteria from Vinegar Fermentation May 2003 Journal of Fluorescence 13(3):261-265

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Antibody-Direct Epifluorescent Filter Technique and

Close mobile search navigation. Article navigation. Volume 59, Issue 10

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Antibody-direct epifluorescent filter technique for rapid

Oct 01, 1994 Rapid membrane filtration-epifluorescent microscopy technique for direct enumeration of bacteria in raw milk. Appl Environ Microbiol. 1980 Feb; 39 (2):423–429. [Europe PMC free article] [Google Scholar] Pettipher GL, Rodrigues UM. Rapid enumeration of microorganisms in foods by the direct epifluorescent filter technique. Appl Environ Microbiol.

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DEFT Direct Epifluorescence Filter Technique AcronymAttic

Over 3 million unverified definitions of abbreviations and acronyms in Acronym Attic. For verified definitions visit AcronymFinder All trademarks/service marks referenced on this site are properties of their respective owners.

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Developments in rapid methods for detection of agents of

The application of techniques such as the direct epifluorescent filter technique (DEFT), electrical impedance or conductance measurements, hydrophobic grid membrane filters (HGMFs), genetically engineered luminescent bacteria, DNA probes, polymerase chain reaction, monoclonal antibodies, and identification kits and systems, to the detection of foodborne microbial pathogens is described.

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Status and Future of the Direct Epifluorescent Filter

The direct epifluorescent filter technique (DEFT) was originally developed for counting bacteria in raw milk at the National Institute for Research in Dairying, Reading (Pettipher et al. 1980; Pettipher 1983). The method, which uses membrane filtration and epifluorescence microscopy, takes less than 30 min to complete and does not suffer from

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Rapid Enumerationof Microorganisms Foods bythe Direct

Rapid EnumerationofMicroorganisms in Foodsbythe Direct Epifluorescent Filter Technique GRAHAML. PETTIPHER*ANDUBALDINAM. RODRIGUES NationalInstituteforResearch in Dairying, Shinfield, ReadingRG29AT, England Received 12 April 1982/Accepted 7 June 1982

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Direct epifluorescent filter technique (DEFT). Use in

Direct epifluorescent filter technique (DEFT). Use in assesment of microbiological quality of water. Foreign Title : Direkte epifluorescens filter teknik (DEFT-metoden). Anvendelse til vurdering af mikrobiologisk vandkvalitet. Author(s) : Boisen, F.

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Improved Direct Epifluorescent Filter Technique for Rapid

Improved Direct Epifluorescent Filter Technique for Rapid Bioburden Control in Intravenous Solutions. Jose Maria Esteban, Mariano Leiva Diaz, Fabian Soriano and Enrique Marin. PDA Journal of Pharmaceutical Science and Technology September 1992, 46 (5) 146-149; Article

More

Review: The direct epifluorescent filter technique

The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the method are discussed.

More

Status and Future of the Direct Epifluorescent Filter

The direct epifluorescent filter technique (DEFT) was originally developed for counting bacteria in raw milk at the National Institute for Research in Dairying, Reading (Pettipher et al. 1980; Pettipher 1983). The method, which uses membrane filtration and epifluorescence microscopy, takes less than 30 min to complete and does not suffer from

More

Antibody-Direct Epifluorescent Filter Technique and

Close mobile search navigation. Article navigation. Volume 59, Issue 10

More

Antibody-direct epifluorescent filter technique for rapid

Oct 01, 1994 Rapid membrane filtration-epifluorescent microscopy technique for direct enumeration of bacteria in raw milk. Appl Environ Microbiol. 1980 Feb; 39 (2):423–429. [Europe PMC free article] [Google Scholar] Pettipher GL, Rodrigues UM. Rapid enumeration of microorganisms in foods by the direct epifluorescent filter technique. Appl Environ Microbiol.

More

Direct epifluorescent filter technique (DEFT). Use in

Direct epifluorescent filter technique (DEFT). Use in assesment of microbiological quality of water. Foreign Title : Direkte epifluorescens filter teknik (DEFT-metoden). Anvendelse til vurdering af mikrobiologisk vandkvalitet. Author(s) : Boisen, F.

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Antibody-direct epifluorescent filter technique for rapid

Artificially inoculated Escherichia coli O157:H7 was directly enumerated in ground beef and beef exudate, without enrichment or selection, by the antibody-direct epifluorescent filter technique (Ab-DEFT). The total assay time of the Ab-DEFT was less than 1 h.

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AbstractThe direct epifluorescent filter technique (DEFT

The direct epifluorescent filter technique (DEFT) is a new rapid method which uses membrane filtration and epifluorescent microscopy for counting in milk. A collaborative trial of the DEFT was conducted between six laboratories. Each laboratory obtained a highly significant relationship between the DEFT count and plate count with a correlation

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MICROBIAL TESTING PROCEDURES

Called the Direct Epifluorescent Filter Technique (DEFT), this method uses dyes and fluorescent microscopy to rapidly enumerate bacteria on a filter. 2. This procedure includes a pre-filtering step to remove large food particles (-if necessary-5µm filter used) and then the filtrate is treated with a detergent and a protease to degrade somatic

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DEFT Direct Epifluorescence Filter Technique AcronymAttic

Over 3 million unverified definitions of abbreviations and acronyms in Acronym Attic. For verified definitions visit AcronymFinder All trademarks/service marks referenced on this site are properties of their respective owners.

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Rapid methods of detection of food borne pathogens

Jan 16, 2018 Microscopic methods: 1)Direct Epifluorescent Filter Technique 2)Flow Cytometry 3)Solid-Phase Cytometry 15. Flow Cytometry -Flow cytometry quantitatively measures optical characteristics of cells when they are forced to pass individually through a beam of light. -Fluorescent dyes can be used to test the viability and metabolic state of

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Microbiological Examination of Foods: 7 Methods

The direct epifluorescent filter technique or DEFT is a microscopy technique which has been applied to the enumeration of micro-organisms in a range of foods, although it was originally developed for estimating bacterial counts in raw milk. The technique was developed in response to the need for a rapid method for judging the hygienic quality

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Acridine orange Wikipedia

Direct epifluorescent filter technique (DEFT) using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange in clinical applications has become widely accepted, mainly focusing on highlighting bacteria in blood cultures. Past and present studies comparing acridine orange staining

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DEFT Direct Epifluorescent Filter Technique in Medical

"Direct Epifluorescent Filter Technique" can be abbreviated as DEFT. Q: A: What is the meaning of DEFT abbreviation? The meaning of DEFT abbreviation is "Direct Epifluorescent Filter Technique". Q: A: What is DEFT abbreviation? One of the definitions of DEFT is "Direct Epifluorescent Filter Technique

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